Test Method

Logan Laboratories uses LC-MSMS Technology

Logan Laboratories uses the most current technology available with the most up-to-date method. For urine drug confirmation testing, using LC/MS/MS technology over other testing methods provides:

  • Reduced labor (simplified sample prep)
  • Lower detection limits
  • Better sensitivity and selectivity
  • Less analysis time
  • Robust quantitative and qualitative analysis

Limitations of Other Testing Methods:

LC-UV

  • Limited specificity and sensitivity
  • Variability of UV spectra
  • Many compounds have little to no UV absorbance

GC-MS

  • Increased sensitivity and specificity (Compared to LC-UV)
  • Cannot detect non-volitile, polar, and thermally labile compounds
  • Requires lengthy sample preparation (hydrolysis/derivatization)
  • High reproducibility of generated mass spectra

Until recently, GCMS was the “Gold Standard” for urine drug confirmation testing, however, there have been several long-standing issues with GCMS recently overcome with the advancement of LCMSMS technology applied to this field.  These issues have been:

  • Sample prep is extensive and expensive
  • Does not cover many compounds – especially new drugs
  • Detection limits are not sufficient for many new more potent drugs
  • Cannot separate similar drugs easily
  • Cannot detect low concentration drugs in the presence of much higher concentrations

URINE DRUG CONFIRMATION  – LCMSMS LOQ/CUTOFFs

ANALYTE NAME

Logan Lab Cut Off (ng/ml)

6-MAM (A Heroin Metabolite)

15

7-Amino-Clonazepam

25

Alpha-OH-Alprazolam

25

Alprazolam

25

Amitriptyline

100

Amphetamine

100

Benzoylecgonine (A Cocaine metabolite)

50

Buprenorphine 10

Carisoprodol

100

Codeine

25

EDDP  (A Methadone metabolite)

25

Fentanyl

1

Gabapentin

500

Hydrocodone

25

Hydromorphone

25

Ketamine

50

Lorazepam

25

MDMA (Ecstasy)

50

Meperidine

25

Meprobamate

100

Methadone

25

Methamphetamine

250

Morphine

25

Naloxone

15

N-Desmethyl Tapentadol

25

Norbuprenorphine

10

Nordiazepam

25

Norfentanyl

1

Nor-Ketamine

50

Normeperidine

25

Nortriptyline

100

Oxazepam

25

Oxycodone

25

Oxymorphone

25

Phencyclidine (PCP)

5

Pregabalin

500

Ritalinic Acid

100

Tapentadol

25

Temazepam

25

THC-COOH

40

Tramadol

25

Zolpidem

10

Zolpidem Phenyl-4-carboxyl

25